The replication origin of pSC101: Replication properties of a segment capable of autonomous replication.
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چکیده
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15 صفحه اولMechanism of recruitment of DnaB helicase to the replication origin of the plasmid pSC101.
Although many bacterial chromosomes require only one replication initiator protein, e.g., DnaA, most plasmid replicons depend on dual initiators: host-encoded DnaA and plasmid-encoded Rep initiator protein for replication initiation. Using the plasmid pSC101 as a model system, this work investigates the biological rationale for the requirement for dual initiators and shows that the plasmid-enco...
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To investigate the role of binding sites for Rep initiation protein in the replication of pSC101, a series of plasmids was constructed which carried different combinations of mutations in three binding sites within the minimal origin of replication. Mutation of all three sites reduced the affinity of purified Rep protein for the origin by 100-fold, as measured by a competition binding assay. Mu...
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ژورنال
عنوان ژورنال: The Journal of General and Applied Microbiology
سال: 1984
ISSN: 1349-8037,0022-1260
DOI: 10.2323/jgam.30.347